Microplate readers include the m3, m4, m5, and m5e models. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection. Spectramax id3 and id5 multimode microplate readers. Spectramax m3, m4, m5, and m5 e multimode microplate. Manuals, cables, power cords, software, drivers, accessories, etc are not included unless pictured or noted in the description. Plates are used to validate optical performance of spectramax m3, m4, and. Spectramax m3 microplate reader from molecular devices llc. Molecular devices spectramax m2 multimode microplate reader. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information.
Spectramax m3 multimode microplate reader, see page 122. Softmax pro data acquisition and analysis software user guide. User manual spectramax m3 m4 m5 m5e software manual softmax pro this entry was posted in equipment, facility news, resources and tagged fluorescence plate reader, plate reader, protein concentration, shared equipment, spectramax m5 plate reader on may 16, 2014 by tania. For fluorescence intensity assays, users can select from top or bottomread modes for improved sensitivity in solution and cellbased assays. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. In no event will mdc be liable to the user for any damages, including any lost profits, lost savings, or other. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence. Molecular devices spectramax id3 multimode microplate reader. With the spectramax minimax 300 imaging cytometer, industryleading softmax pro software, and user. Spectramax m3, m4, m5, and m5 e multimode microplate readers. Spectramax i3 spectratest fl1 protocol softmax pro.
Read online spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link book now. Established in 1911, the university of tennessee health science center aims to improve human health through education, research, clinical care and public service. Molecular devices just launched spectramax id3 multimode microplate reader is the cornerstone of complete laboratory solution helping to expand the boundaries of research. The pathcheck sensor can extend the instrument linearity by a factor of three or more. The spectramax i3x microplate reader from molecular devices evolves with your future research needs and offers an unlimited breadth of applications the spectramax i3x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality. Many still advocate for hps and mh grow lights, but in recent years, led grow lights have become much more advanced than when they first came into the market. This revolution in modularity makes nocompromise detection the norm. The ut health science center campuses include colleges of dentistry, graduate health sciences, health professions, medicine, nursing and pharmacy. To view the softmax pro user guide, the formula reference guide, or the. Molecular devices spectramax m5 multimode microplate. Spectramax microplate spectrophotometer operators manual.
Patient care, professional education and research are carried out at hospitals and. Using the molecular devices test plate to calibrate the. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. We therefore gathered salient information about many of the instruments available to date. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Userupgradeable applications userexchangeable detection modules expand the systems detection capabilities to include timeresolved fluorescence, htrf, fast kinetics with injectors, and western blot detection.
The spectramax 190 reader is the tool for dna analysis. Rapid, accurate dna, rna, and protein quantitation in a one touch, full spectrum micro volume absorbance reader. The spectramax i3x multimode microplate reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Spectramax id3 multimode microplate reader molecular. With optimized reagents and the industryleading data acquisition and analysis tool softmax pro 7 software, the spectramax id3 allows customization of workflow to. Molecular devices spectramax id3 multimode microplate. Spectramax plus 384 microplate reader manual conquer. Compatible automation solutions for spectramax readers. Software for the test plate must be installed on the control computer before the plate can be used. For more detailed information and technical support of molecular devices instruments or software. Spectramax i3 multimode platform a flexible, userupgradeable microplate detection system key features the spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities.
Softmax pro software user guide marine biological laboratory. Optics mirrored optics focus the light into the sample volume, and cutoff filters are used to. The spectramax m2 and m2e readers provide two detection modes in one platform, and offer the the added functionality of bottomread fluorescence for cellbased assays m2e. Specifications see manual for details does uv and visible absorbance, and fluorescence intensity measurements on microplates and. Softmax pro software user guide biological discovery in. Luciferase detection instrumentation guide thermo fisher. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type. Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a. The spectramax m3 microplate reader is the standard for uvvisible multimode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6, 12, 24, 48, 96, and 384well microplates.
The upgradeable spectramax m5 multimode microplate reader comes with the standard five modes of detection with the upgrade option to add htrf at a later time. The following document is intended to demonstrate setup of this instrument. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Molecular devices spectramax m3 multi mode microplate reader.
Microplate readers, multimode, spectramax m series vwr. Molecular devices spectramax 190 microplate reader includes softmax. Spectramax m series multimode microplate readers 7 the spectradrop microvolume microplate offers the ability to use as little as 2l samples with 24 or 64well plates. Catchpoint simplestep elisa kits give users a 90minute elisa workflow, a nearly twothirds reduction in time compared to conventional elisas. Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Molecular devices spectramax m3 multimode microplate reader 90 day warranty. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to. All spectramax m series multimode microplate readers measure uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. Optics mirrored optics focus the light into the sa mple volume, and cutoff filters are used to.
Operators manual molecular devices spectramax 190 absorbance plate reader. Introduction the spectramax m2 and spectramax m2 e multidetection readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well. Spectramax m2m2e user guide university of michigan. Future proof your discoveries and let the spectramax i3x reader evolve with your research. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. Apr 07, 2015 molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. Setup guide on the molecular devices spectramax m5m5e. The spectramax m2 and spectramax m2e multi detection readers are monochromator based microplate readers that have 6 well to 384 well. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. The broad array of spectramax i3x detection cartridges enables the user. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. Spectramax m3, m4, m5, and m5 e multimode microplate readers user guide 01120115 f july 2010. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform.
Spectramax m series multimode microplate readers molecular. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectramax m3 reader spectramax m4 reader spectramax m5 reader spectramax m5e reader detection modes. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. With optimized reagents, option to operate the readers. Unit powers up but is not tested for any analytical application, we dont run any liquid. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. Using the molecular devices test plate to calibrate the spectramax reader. Spectramax m3 multimode microplate reader, molecular devices. Spectramax m series microplate readers, cuvette port, iq oq. Spectramax plus 384 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax plus 384 control panel are shown in boxed helvetica type. Spectramax m3, m4, m5, and m5e multimode microplate reader. For very concentrated samples, use a minimum volume. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays.
Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. Spectramax 190 microplate spectrophotometer operators manual iii labor and parts to obtain warranty service during the applicable warranty period, you must take the product or deliver the product properly packaged in the original shipping materials and carton to an authorized molecular devices corporation service facility. Spectramax i3 spectratest lm1 protocol softmax pro. Spectramax plus 384 microplate reader manual conquer scientific. These robust readers have been placed in labs from antarctica to the. Spectramax led lights minimize cost, maximize light. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch. These settings are also valid for the spectramax m3m4, flexstation 3 multimode. Spectramax m3 multimode microplate reader, see page 202. The spectramax m5 multimode microplate reader delivers single mode reader performance in one multimode reader package. The cuvette chamber spectramax plus 384 only figure 1.
Spectramax m5 microplate reader from molecular devices llc. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects. The spectramax m2 is a highly flexible dual monochromator, multimode microplate reader with full spectral range detection for cuvettes and 6 to 384 well microplates. Molecular devices spectramax i3 manual nova scotia. Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Spectramax i3 multimode detection platform youtube. Multimode microplate reader spectra max m3 climate impacts. Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. The ability to detect 16 ngwell of dna and to quantitate 50 ng of dna provides the same sensitivity as a spectrophotometer. All books are in clear copy here, and all files are secure so dont worry about it.
The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. Softmax pro software has been integrated by many leading robotics and. The m2 can scan across a range of wavelengths in 1 nm increments and a single read command can run a combined absorbance and fluorescence intensity assay. Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16. Download spectramax m3, m4, m5, and m5e multimode microplate. Many still advocate for hps and mh grow lights, but in recent years, led grow lights have become. In this user guide, all references to spectramax multimode. Dec 03, 20 the spectramax minimax 300 imaging cytometer enables cell visualization and cellbased analysis on the spectramax i3 multimode microplate reader. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence. Download spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link or read online here in pdf. The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Spectramax m5 plate reader proteomics and mass spectrometry. Read online spectramax m3, m4, m5, and m5e multimode microplate.
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